Δράση τροποποιητών βιολογικής απόκρισης και αντικαρκινικών φαρμάκων στη δομή και λειτουργία ενζύμων σταθερότητας μηνύματος RNA: ο ρόλος τους στη βιολογία του καρκίνου και η σημασία στη διάγνωση και θεραπεία

Abstract

THE FINAL PATHWAY OF ALL CANCER GROWTH IS THROUGH AN ABERRANT NUCLEIC ACID SEQUENCE.LEADING TO A MORE RAPID CELL GROWTH.THE PRESENT STUDY AIMS TO THE EXAMINATION OF ANY POSSIBLE ACTION OF THREE WIDELY USED ANTICANCER DRUGS,5-FLUOROURACIL (5-FU), RECOMBINANT INTERFERON-A (RIFIN-A)AND TAMOXIFEN (TAM) AH THE POST-TRANSCRIPTIONAL LEVER.THE MONITORING OF ACTION AT THIS LEVER IS PERFORMED THROUGH POLY (A) POLYMERASE (PAP),THE ENZYME WHICH ADDS THE POLY (A) TAIL.HELA AND WISH CELLS WERE EXPOSED TO THESE DRUGS AND SUBSEQUENT PAP ACTIVITY WAS MONITORED USING NON-SPECIFIC ASSAY AND THE LEVELS OF PAP ISOFORMS WITH IMMUNOBLOTTING.5-FU AND R/FN-A REDUCED THE LEVELS OF TWO MAIN ISOFORMS FROM HELA CELLS WITH MD.WEIGHTS 100 AND 106 KDA,JUST AFTER 15' OF DRUG PRESENCE.TAM DID NOT AFFECTED PAP ISOFORMS, BUT PROVOKED MAXIMUM ENZYMIC ACTIVITY REDUCTION.EXPERIMENTS WITH PHOSPHATASES AND PHOSPATASE INHIBITORS REVEALED THAT DEPHOSPORYLATION IS ONE POSSIBLE MECHANISM OF THIS ACTION.5-FU PRESENCE WAS ACCOMPANIED WITH APOPTOTIC PHENOMENA IN HELA BUT IN WISH CELLS.THE PREVIUS DATA SUPPORT THEIR POST TRANSCRIPTIONAL LEVEL SEEMS TO BE INFLUENCED BY ANTICANCER DRUG PRESENCE VIA DEPHOSPHORYLATION AND THAT PAP COULD DE USED AS AN EARLY BIOMARKER OF CELL TRANSFORMATION AND RESPONSE TO CHEMOTHERAPY.

OBJECTIVE: THE PURPOSE OF THIS STUDY WAS TO ASSESS THE NEUROPSYCHOLOGICAL RISK OF OCCUPATIONAL EXPOSURE TO LIQUID OR VAPOUR FUEL IN CONDITIONS OF ATMOSPHERIC POLLUTION DUE TO HEAVY TRAFFIC. METHOD: 38 PETROL STATION ATTENDANTS,51 TAXI DRIVERS AND 46 BUS DRIVERS AND 46 CONTROLS WERE TESTED USING STANDARD NEUROPSYCHOLOGICAL TESTS,IN ORDER TO ASSESS THE LEVER OF NEUROPSYCHOLOGICAL FUNCHIONING AND TO BE TEST THE EFFECTS OF LONG-TERM NEUROTOXIC EXPOSURE IN THE MEASURMENTS OF THE HIGHER LEVER FUNCTION.RESULTS: THE LEAD LEVELS IN THE BLOOD OF ALL THOSE PARTECIPATING IN THE STUDY HAVE BEEN FOUND TO BE BELLOW THE SUGGESTED SAFETY LEVELS.OVERALL IN THE N.TESTS NO DIFFERENCES WERE FOUND BETWEEN THE GROUPS AND TAKEN TOGETHER.IN CERTAIN N.TESTS LEAD APPEARS TO AFFECT THE SPEED OF PROCESSING VISUAL INFORMATION,MENTAL TRACKING AND VISUO-MOTOR COORDINATION.CONCLUSIONS:LONG TERM EXPOSURE TO EVEN LOW LEVELS OF LOAD CONITITUTS A NEUROTOXIC THREAT WITH ADVERSE REPARCUSSIONS IN THE NEUROPSYCHOLOGICAL FUNCTIONING.THE FINDINGS STRENGTHEN THE VIEW THAT THERE ARE NO "SAFETY" LEVELS OF EXPOSURE TO LOAD.