Μελέτη της επίπτωσης της χορήγησης βλαστοκυττάρων από λιπώδη ιστό στην βιωσιμότητα ολικού πάχους μοσχευμάτων δέρματος σε διαβητικούς και μη διαβητικούς επίμυες και ο συσχετισμός της όποιας επιδράσεως με την έκφραση των παραγόντων VEFG και TGFb3

Abstract

Background: Skin grafts are frequently used for a variety of indications in plastic andreconstructive surgery. heir necrosis is a common complication while differenttherapies have been proposed. Currently, adipose-derived stem cells (ASCs) holdgreat promise for their angiogenic potential and role during tissue repair. In this study,autologous transplantation of ASCs was usedin skin grafts in diabetic and nondiabeticrats to determine if it increases angiogenesis, skin graft survival and woundhealing.Methods: ASCs were isolated, cultured, labeled with fluorescent dye and injectedunder full thickness skin grafts in twenty rats (group 1 and group 3, non-diabetic anddiabetic respectively, ten rats in each group), while twenty others served as controls(group 2 and group 4, non-diabetic and diabetic respectively, ten rats in each group).immunohistochemistry. Additionally, immunohistochemical staining intensity ofvascular endothelial growth factor (VEGF) and transforming growth factor b3(TGFb3) was assessed in all grafts.Results: Mean area of graft necrosis was significantly less in group 1 than group 2(6.12% vs. 32.62%, p<0.01). Statistically significant increase of microvessel density,collagen density, VEGF and TGFb3 expression was noted in group 1 compared withgroup 2 (all: p<0.01). Additionally, mean area of graft necrosis was significantly lessin group 3 than group 4 (7.49% vs. 39.67%, p<0.001). Statistically significant30increase of capillary density, collagen intensity, VEGF and TGFb3 expression wasnoted in group 3 compared with group 4.Conclusions: These findings suggest that autologous ASCs transplantation increasesfull-thickness skin graft survival and shows promise for use in skin graft surgery. Thismight be both due to in situ differentiation of ASCs into endothelial cells and ASCsincreased secretion of growth factors such as VEGF and TGFb3 that enhanceangiogenesis and wound healing. This might represent a novel therapeutic approach inskin graft surgery which is particularly useful for diabetic wounds.