In vitro μελέτη της απελευθέρωσης νεώτερων αντιμικροβιακών ουσιών από κυλίνδρους οστικού τσιμέντου φωσφορικού ασβεστίου και από κυλίνδρους οστικού τσιμέντου ακρυλικού ασβεστίου

Abstract

SUMMARY Therapeutic approach of chronic osteomyelitis remains a problem in every day clinical practice mainly due to adverse side effects (in IV or pos therapy). Local antibiotic delivery systems have evolved in an attempt to reduce the extended use of IV or pos therapy. AIM : The evaluation of in vitro release of teicoplanin and moxifloxacin (in 3% concentration) impregnated in acrylic and phosphate bone cement respectively. METHODS: 10 specimens (5 for each antibiotic bone cement) are prepared in sterile vials. Mueller Hinton broth which is added in every vial covering the free surface of the cement , is replaced every 24 hours. The daily obtained samples have been kept in -80C until antibiotics concentrations are determined. ( bioassay and HPLC). RESULTS : Moxifloxacin was eluted at very high concentrations within the first two weeks. Concentrations remained above the MICs of the most common pathogens related to bone infection . It is concluded that both cements may allow the adequate in vitro elution of moxifloxacin . The latter results support their application in experimental models of osteomyelitis. _ Teicoplanin concentrations was 10 times higher within the first week from acrylic bone cement compared to concentrations eluted from NORIAN-teicoplanin .The elution of teicoplanin from acrylic bone cement lasted for 24 days. Concentrations remained above the MICs of the most common pathogens related to bone infection, although lasted less than the moxifloxacin bone cement system . 138 CONCLUSIONS The release of moxifloxacin from both acrylic and phosphate bone cement is promising and should be tested in in vivo models.